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KMID : 1024420090130040251
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Food Engineering Progress
2009 Volume.13 No. 4 p.251 ~ p.261
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Effect of DIS Process on in vitro Physiological Functionality of Aloe vera Gel
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Kim Sung-A
Lee Shin-Young
Baek Jin-Hong
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Abstract
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In vitro physiological functions such as jack bean (Canavalia ensiformis) urease inhibitory activity and retarding effect of glucose/bile acid of Aloe vera gel concentrated by the optimized DIS (Dewatering Impregnation & Soaking) process conditions were examined. Urease inhibitory activity of DIS aloes ranged from 84.6 to 94.4%, which was similar to or higher than 86.3% of fresh aloe. Also, urease inhibitory activity of DIS aloes was maintained at initial levels after heat treatment (90℃, 10 min.) and drying treatment (freeze or hot air drying). Urease inhibition pattern from Lineweaver-Burk plot indicated general non-competitive inhibition, and inhibition constants (K(IE) and K(IES)) of DIS aloes were 41-149 and 87-163 μL/mL, respectively. DIS(glucose) and DIS(polyethylene glycol) exhibited the highest retarding effect of glucose and bile acid. Their retarding effects were about 1.6 and 1.8 folds higher than that of fresh aloe after 0.5 and 1 hr of the dialysis, respectively. Conclusively, the above in vitro physiological functions of Aloe vera gel concentrated by DIS process suggested that aloe products treated with DIS would have the potential benefits for protection against Helicobacter pylori and reduction of blood glucose and cholesterol levels.
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KEYWORD
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dewatering impregnation & soaking, Aloe vera gel, urease inhibition, glucose/bile acid retarding effect
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